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Objective:To study the inhibitory effect of bone marrow mesenchymal stem cells (MSC) on the proliferation of allogeneic T lymphocytes in patients with acute leukemia and its mechanism. Methods: 30 patients with acute myeloid leukemia ( AML) ,30 patients with acute lymphoblastic leukemia ( ALL) ,and 30 healthy subjects ( healthy group) were selected. MSC cells were isolated and cultured in three groups. The expression level of MSC cells in three groups were determined by MSC assay. The inhibitory effect of MSC on the proliferation of T cells was detected by Transwell assay. Results: AML group supernatant TGF-β1,HGF levels were significantly lower than the ALL group and the healthy group (P0. 05). AML,ALL,MSC cells secrete cytokines healthy group of T lymphocyte proliferation still had a significant inhibitory effect, inhibition of differences in the case of contact and not in direct contact co-culture of T lymphocyte proliferation was not statistically sig-nificant (P> 0. 05); after joining the MSC,T cell proliferation in the three groups was significantly inhibited upon addition of anti-TGF-β1 antibody,anti-HGF antibody after T cell proliferation effective than a simple reversal join MSC (P<0. 05). Conclusion:MSC for acute leukemia patients inhibit allogeneic T lymphocyte proliferation,functioning principle may be related to the secretion of cyto-kines.
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Objective To investigate the expression of hepatocyte growth factor (HGF)and vascular endothelial growth factor (VEGF)in acute myeloid leukemia(AML) and its significance in the progress of AML.Methods Serum levels of HGF and VEGF in AML patients,including 25 de novo cases,17 cases with complete remission( CR),16 cases without remission and 12 cases with relapse,were measured by enzyme-linked immunosorbent assay( ELISA ).The data were compared with those of normal controls.Results The serum levels of HGF were( 1357.29 ± 358.64)ng/L in de novo patients,(1175.93 ± 306.71 )ng/L in patients without remission and( 1261.21 ± 340.83 )ng/L in patients with relapse,which were significantly higher than those in normal control(232.62 ± 99.13 ) ng/L and CR patients ( 256.65 ± 94.32 ) ng/L( F =78.35,P ＜ 0.01 ).The serum levels of VEGF were ( 253.84 ± 49.14 ) ng/L in de novo patients,( 245.87 ± 54.68 ) ng/L in patients without remission and(264.75 ± 62.52) ng/L in patients with relapse,which were significantly higher than those in normal control ( 97.61 ± 16.19 ) ng/L and CR patients ( 99.76 ± 15.93 ) ng/L ( F =68.65,P ＜ 0.01 ).Compared with de novo group,the serum levels of HGF and VEGF in CR patients decreased significantly but showed no significant difference from those in normal controls(P ＞0.05).The expression of HGF and VEGF in acute myeloid leukemia was positively correlated with each other ( r =0.49,P ＜ 0.05 ).Conclusion The expression of HGF and VEGF was closely related with the incidence and development of acute myeloid leukemia.Preventing HGF expression may be a possible strategy for leukemia therapy in the future.
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Objective To study the effect of the plasma exchange in the treatment of RITP with 2∶2∶1 exchange liquid.Methods 32 cases of RITP were treated with plasma exchange using CS 3000 blood cell separator.Results Plasma exchange could reduce the levels of harmful substances like PA Ig,PA C 3,CIC and raise the levels of platelet by 3 times.Conclusion Plasma exchange is effective in the treatment of RITP with 2∶2∶1 exchange liquid,with reliable outcome.